大肠杆菌启动子的基本结构：两个共有序列之间间隔长度对启动子功能的影响。
Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function.
作者信息
Aoyama T, Takanami M, Ohtsuka E, Taniyama Y, Marumoto R, Sato H, Ikehara M
出版信息
Nucleic Acids Res. 1983 Sep 10;11(17):5855-64. doi: 10.1093/nar/11.17.5855.
A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the -10 region.
构建了一个在 -35 和 -10 区域具有共有序列（TTGACA 和 TATAAT）的启动子，并在两个限制性位点独立改变两个共有序列之间的距离。在体外转录系统中，无论空间调整的位点如何，在 17 个碱基对的间隔长度处观察到最大活性。还提供了证据表明转录起始点由 -10 区域的共有序列固定。
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