脱氧核糖核酸及相关化合物的研究。V. 使用一种新型磷酸化试剂通过磷酸三酯法合成含有启动子理想普里布诺序列的十五聚体双链体。
Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.
作者信息
Ohtsuka E, Taniyama Y, Marumoto R, Sato H, Hirosaki H, Ikehara M
出版信息
Nucleic Acids Res. 1982 Apr 24;10(8):2597-608. doi: 10.1093/nar/10.8.2597.
Abstract
A phosphorylating reagent o-chlorophenyl phosphoro-p-anisi-dochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as 3'-terminal units for the synthesis of oligonucleotide blocks. By condensation of these oligonucleotide blocks the partially complementary deoxypentadecanucleotides dAGCTTATAATGC-TCG and dAGCTCGAGCATTATA, which contained the ideal Pribnow sequence TATAATG, were synthesized.
摘要
合成了一种磷酸化试剂邻氯苯基对茴香基磷酰二氯，用于对N，5'-保护的脱氧核苷的3'-羟基进行磷酸化。这些核苷酸用作合成寡核苷酸片段的3'-末端单元。通过这些寡核苷酸片段的缩合，合成了部分互补的脱氧十五聚核苷酸dAGCTTATAATGCTCG和dAGCTCGAGCATTATA，它们包含理想的Pribnow序列TATAATG。
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