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脱氧核糖核酸及相关化合物的研究。V. 使用一种新型磷酸化试剂通过磷酸三酯法合成含有启动子理想普里布诺序列的十五聚体双链体。

Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.

作者信息

Ohtsuka E, Taniyama Y, Marumoto R, Sato H, Hirosaki H, Ikehara M

出版信息

Nucleic Acids Res. 1982 Apr 24;10(8):2597-608. doi: 10.1093/nar/10.8.2597.

DOI:10.1093/nar/10.8.2597
PMID:7079181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320636/
Abstract

A phosphorylating reagent o-chlorophenyl phosphoro-p-anisi-dochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as 3'-terminal units for the synthesis of oligonucleotide blocks. By condensation of these oligonucleotide blocks the partially complementary deoxypentadecanucleotides dAGCTTATAATGC-TCG and dAGCTCGAGCATTATA, which contained the ideal Pribnow sequence TATAATG, were synthesized.

摘要

合成了一种磷酸化试剂邻氯苯基对茴香基磷酰二氯，用于对N，5'-保护的脱氧核苷的3'-羟基进行磷酸化。这些核苷酸用作合成寡核苷酸片段的3'-末端单元。通过这些寡核苷酸片段的缩合，合成了部分互补的脱氧十五聚核苷酸dAGCTTATAATGCTCG和dAGCTCGAGCATTATA，它们包含理想的Pribnow序列TATAATG。

相似文献

1
Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.脱氧核糖核酸及相关化合物的研究。V. 使用一种新型磷酸化试剂通过磷酸三酯法合成含有启动子理想普里布诺序列的十五聚体双链体。
Nucleic Acids Res. 1982 Apr 24;10(8):2597-608. doi: 10.1093/nar/10.8.2597.
2
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.通过磷酸三酯中间体合成寡核苷酸的新有效方法。
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675.
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A rapid deprotection procedure for phosphotriester DNA synthesis.一种用于磷酸三酯DNA合成的快速脱保护程序。
Nucleic Acids Res. 1984 Sep 11;12(17):6853-9. doi: 10.1093/nar/12.17.6853.
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Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.硅胶：用于寡核苷酸固相磷酸三酯合成的一种改进载体。
Nucleic Acids Res. 1982 Nov 25;10(22):7439-48. doi: 10.1093/nar/10.22.7439.
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A novel class of condensing reagents in phosphodiester oligodeoxyribonucleotides synthesis. Application of the constituents of free terminal carboxy oxytocine gene.磷酸二酯寡脱氧核糖核苷酸合成中的一类新型缩合试剂。游离末端羧基催产素基因成分的应用。
Nucleic Acids Symp Ser. 1980(7):23-37.
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Nucleic Acids Symp Ser. 1980(7):243-57.
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Nucleic Acids Res. 1984 Feb 24;12(4):2091-110. doi: 10.1093/nar/12.4.2091.
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Use of phosphorus oxychloride in synthesizing nucleotides and oligonucleotides.三氯氧磷在核苷酸和寡核苷酸合成中的应用。
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Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA.人胰岛素基因的合成。第二部分。改良磷酸三酯法的进一步改进以及构成人胰岛素B链和微型cDNA的17个脱氧核糖寡核苷酸片段的合成。
Nucleic Acids Res. 1979 Dec 20;7(8):2199-212. doi: 10.1093/nar/7.8.2199.

引用本文的文献

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A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support.一种新型缩合试剂，1-(2,4,6-三异丙基苯磺酰基)-5-(吡啶-2-基)四唑化物及其在聚合物载体上合成λ cro结合十七聚核苷酸中的应用。
Nucleic Acids Res. 1982 Oct 25;10(20):6235-41. doi: 10.1093/nar/10.20.6235.
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Studies on transfer ribonucleic acids and related compounds. XLV. Block condensation of ribooligonucleotides containing 2'-O-tetrahydrofuranyl-5'-O-dimethoxytritylnucleosides.转移核糖核酸及相关化合物的研究。XLV。含2'-O-四氢呋喃基-5'-O-二甲氧基三苯甲基核苷的核糖寡核苷酸的阻断缩合反应。
Nucleic Acids Res. 1983 Mar 11;11(5):1325-35. doi: 10.1093/nar/11.5.1325.
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Nucleic Acids Res. 1983 Sep 10;11(17):5855-64. doi: 10.1093/nar/11.17.5855.
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Synthesis of a gene for human growth hormone and its expression in Escherichia coli.人生长激素基因的合成及其在大肠杆菌中的表达。
Proc Natl Acad Sci U S A. 1984 Oct;81(19):5956-60. doi: 10.1073/pnas.81.19.5956.

本文引用的文献

1
Simplications in the synthesis of short oligonucleotide blocks.短寡核苷酸片段合成中的简化方法。
Nucleic Acids Res. 1980 Nov 25;8(22):5461-71. doi: 10.1093/nar/8.22.5461.
2
Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.一些与肟离子促进寡核苷酸芳基酯解封闭相关的观察结果。
Nucleic Acids Res. 1981 Sep 25;9(18):4611-26. doi: 10.1093/nar/9.18.4611.
3
Studies on transfer ribonucleic acids and related compounds. XL. Synthesis of an eicosaribonucleotide corresponding to residues 35-54 of tRNAfMet from E. coli.转移核糖核酸及相关化合物的研究。XL。对应于大肠杆菌甲硫氨酸起始转运核糖核酸（tRNAfMet）35 - 54位残基的二十碳核糖核苷酸的合成。
Nucleic Acids Res. 1981 Jul 24;9(14):3503-22. doi: 10.1093/nar/9.14.3503.
4
Studies on deoxyribonucleic acids and related compounds. III. Synthesis of oligodeoxyribonucleotides complementary to the anticodon loop of Escherichia coli tRNAfmet by an improved triester method.脱氧核糖核酸及相关化合物的研究。III. 用改进的三酯法合成与大肠杆菌甲硫氨酸起始转运核糖核酸反密码子环互补的寡脱氧核糖核苷酸。
Chem Pharm Bull (Tokyo). 1981 Dec;29(12):3440-8. doi: 10.1248/cpb.29.3440.
5
Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA.使用由合成寡核苷酸引发的DNA聚合酶I来确定噬菌体f1 DNA中的核苷酸序列。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209-13. doi: 10.1073/pnas.70.4.1209.
6
CV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxyribonucleotide corresponding to the nucleotide sequence 31 to 50.简历。酵母丙氨酸转移核糖核酸结构基因的全合成。对应于核苷酸序列31至50的二十碳脱氧核糖核苷酸的化学合成。
J Mol Biol. 1972 Dec 28;72(2):251-88. doi: 10.1016/0022-2836(72)90148-9.
7
The use of nuclease P1 in sequence analysis of end group labeled RNA.核酸酶P1在末端基团标记RNA序列分析中的应用。
Nucleic Acids Res. 1977 Dec;4(12):4091-108. doi: 10.1093/nar/4.12.4091.
8
Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.芳基磺酰四唑，新型偶联试剂以及脱氧核糖寡核苷酸合成三酯法的进一步改进。
Nucleic Acids Res. 1977 Feb;4(2):353-71. doi: 10.1093/nar/4.2.353.