Ohtsuka E, Taniyama Y, Marumoto R, Sato H, Hirosaki H, Ikehara M
Nucleic Acids Res. 1982 Apr 24;10(8):2597-608. doi: 10.1093/nar/10.8.2597.
A phosphorylating reagent o-chlorophenyl phosphoro-p-anisi-dochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as 3'-terminal units for the synthesis of oligonucleotide blocks. By condensation of these oligonucleotide blocks the partially complementary deoxypentadecanucleotides dAGCTTATAATGC-TCG and dAGCTCGAGCATTATA, which contained the ideal Pribnow sequence TATAATG, were synthesized.
合成了一种磷酸化试剂邻氯苯基对茴香基磷酰二氯,用于对N,5'-保护的脱氧核苷的3'-羟基进行磷酸化。这些核苷酸用作合成寡核苷酸片段的3'-末端单元。通过这些寡核苷酸片段的缩合,合成了部分互补的脱氧十五聚核苷酸dAGCTTATAATGCTCG和dAGCTCGAGCATTATA,它们包含理想的Pribnow序列TATAATG。