Chen S, Blanck G, Pollack R E
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5670-4. doi: 10.1073/pnas.80.18.5670.
We have used several inbred mouse strains to examine the role of the 54-kilodalton (kDa) cellular phosphoprotein in transformation by the papovavirus simian virus 40. We have measured the endogenous 54-kDa phosphoprotein in cells obtained from these inbred mouse strains. To study the effect of passage, cell cultures were measured for amount of the 54-kDa phosphoprotein at the 2nd and 12th passages. In the absence of any transforming agent, the amount of endogenous 54-kDa phosphoprotein in early pre-crisis mouse cells varied in a strain-specific way. Transformation frequency varied coordinately with endogenous 54-kDa expression. Mouse strains whose cells produced a high level of endogenous 54-kDa phosphoprotein on passage did not further increase its expression after simian virus 40 transformation.
我们使用了几种近交系小鼠品系来研究54千道尔顿（kDa）细胞磷蛋白在多瘤病毒猿猴病毒40转化过程中的作用。我们测量了从这些近交系小鼠品系获得的细胞中的内源性54-kDa磷蛋白。为了研究传代的影响，在第2代和第12代时测量细胞培养物中54-kDa磷蛋白的量。在没有任何转化剂的情况下，早期危机前小鼠细胞中内源性54-kDa磷蛋白的量以品系特异性方式变化。转化频率与内源性54-kDa表达协同变化。其细胞在传代时产生高水平内源性54-kDa磷蛋白的小鼠品系在猿猴病毒40转化后不会进一步增加其表达。
