人类肿瘤DNA的转化活性。
Transforming activity of human tumor DNAs.
作者信息
Krontiris T G, Cooper G M
出版信息
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1181-4. doi: 10.1073/pnas.78.2.1181.
High molecular weight DNAs of 26 human tumors and tumor cell lines were assayed for the presence of transmissible activated transforming genes by transfection of NIH 3T3 mouse cells. DNAs of two bladder carcinoma cell lines induced transformation with high efficiencies (approximately 0.2 transformant per microgram of DNA), whereas DNAs of the other tumors studied lacked detectable transforming activity. These findings suggest that dominant mutations or gene rearrangements can result in the activation of cellular transforming genes in some human tumors.
通过将DNA转染至NIH 3T3小鼠细胞中，检测了26种人类肿瘤及肿瘤细胞系的高分子量DNA中是否存在可传递的活化转化基因。两种膀胱癌细胞系的DNA能高效诱导转化（每微克DNA约产生0.2个转化体），而所研究的其他肿瘤的DNA则缺乏可检测到的转化活性。这些发现表明，显性突变或基因重排可导致某些人类肿瘤中的细胞转化基因被激活。
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