Glaser R, Boyd A, Stoerker J, Holliday J
Virology. 1983 Aug;129(1):188-98. doi: 10.1016/0042-6822(83)90405-1.
Attempts were made to functionally map antigenic expression of the Epstein-Barr virus (EBV) to specific regions on the EBV genome, using the B95-8 strain. Experiments were performed to map the expression of early antigen (EA), both restricted and diffuse (R and D, respectively), and the EBV nuclear antigen (EBNA), using intact B95-8 DNA, cloned BamHI fragments or Charon 4A fragments. DNA preparations were microinjected into two EBV genome-negative epithelial tumor cell lines. Expression of EBV antigens was monitored using precharacterized human sera, as well as monoclonal antibodies to EA-R and EA-D. The data suggest that EA-R maps to the BamHI H fragment, and EA-D maps to the Charon 4A fragment 7. A previous report that BamHI K is associated with the expression of a nuclear neoantigen tentatively identified as EBNA (W.P. Summers, E.A. Grogan, D. Shedd, M. Robert, C.R. Liu, and G. Miller, Proc. Nat. Acad. Sci. USA 79, 5688-5692, 1982) was also confirmed.
研究人员试图利用B95 - 8毒株，在功能上把爱泼斯坦 - 巴尔病毒（EBV）的抗原表达定位到EBV基因组的特定区域。利用完整的B95 - 8 DNA、克隆的BamHI片段或Charon 4A片段，进行了实验来定位早期抗原（EA，分别为局限型和弥漫型，即R和D）以及EBV核抗原（EBNA）的表达。将DNA制剂显微注射到两种EBV基因组阴性的上皮肿瘤细胞系中。使用预先鉴定的人血清以及针对EA - R和EA - D的单克隆抗体来监测EBV抗原的表达。数据表明，EA - R定位于BamHI H片段，而EA - D定位于Charon 4A片段7。之前一份关于BamHI K与一种暂定为EBNA的核新抗原表达相关的报告（W.P. 萨默斯、E.A. 格罗根、D. 谢德、M. 罗伯特、C.R. 刘和G. 米勒，《美国国家科学院院刊》79, 5688 - 5692, 1982）也得到了证实。
