Stoerker J, Holliday J E, Glaser R
Virology. 1983 Aug;129(1):199-206. doi: 10.1016/0042-6822(83)90406-3.
In a previous study the identification of a region(s) of the Epstein-Barr virus (EBV) genome, which is associated with transformation, was attempted by marker rescue. A transforming EBV was rescued from D98/HR-1 hybrid cells, which contain the non-transforming HR-1 EBV genome, after transfection with specific BamHI and Charon 4A fragments (J. Stoerker and R. Glaser, Proc. Nat. Acad. Sci. USA 80, 1726-1729, 1983). In this study, characterization of the EBV DNA in four human lymphoblastoid cell lines (LCL) transformed with rescued virus was performed. It was found that recombination between the transfected fragments, BamHI H,F,X and the Charon 4A fragment (EB-26-36) which is equivalent to the BamHI H,F,X region, and the endogenous HR-1 EBV genome in the D98/HR-1 cells took place. This recombination resulted in the formation of transforming EBV. The EBV DNA in the four LCLs are similar to each other and to HR-1 EBV DNA. However, the EBV DNA in all four LCLs also contain the U2 region plus additional sequences of B95-8 DNA. The U2 region is deleted in HR-1 EBV DNA which is associated with HR-1 cells and the D98/HR-1 hybrid cells. Thus, transforming activity of the HR-1-like viruses rescued from D98/HR-1 cells was concomitant with the recombination of the 0.26-0.36 region of the EBV genome, suggesting that this region is necessary for at least the initiation of transformation.
在之前的一项研究中，尝试通过标记拯救来鉴定爱泼斯坦-巴尔病毒（EBV）基因组中与转化相关的区域。在用特定的BamHI和Charon 4A片段转染后，从含有非转化性HR-1 EBV基因组的D98/HR-1杂交细胞中拯救出了一种转化性EBV（J. Stoerker和R. Glaser，《美国国家科学院院刊》80，1726 - 1729，1983）。在本研究中，对用拯救出的病毒转化的四个人类淋巴母细胞系（LCL）中的EBV DNA进行了表征。发现转染片段BamHI H、F、X和与BamHI H、F、X区域等效的Charon 4A片段（EB - 26 - 36）与D98/HR-1细胞中的内源性HR-1 EBV基因组之间发生了重组。这种重组导致了转化性EBV的形成。四个LCL中的EBV DNA彼此相似，也与HR-1 EBV DNA相似。然而，所有四个LCL中的EBV DNA还包含U2区域以及B95-8 DNA的额外序列。U2区域在与HR-1细胞和D98/HR-1杂交细胞相关的HR-1 EBV DNA中缺失。因此，从D98/HR-1细胞中拯救出的HR-1样病毒的转化活性与EBV基因组0.26 - 0.36区域的重组相伴，这表明该区域至少对于转化的起始是必需的。
