Bowen-Pope D F, Ross R
Biochem Biophys Res Commun. 1983 Aug 12;114(3):1036-41. doi: 10.1016/0006-291x(83)90666-6.
Human whole blood serum reduces the binding of [125I]labeled mouse epidermal growth factor to cultured human fibroblasts as well as does 70-100 ng/ml mouse epidermal growth factor. However, at most 1-2% of the binding inhibitor detected in human whole blood serum is related to epidermal growth factor--the remaining consists of other factors released during preparation of serum, predominantly the platelet-derived growth factor, which are capable of altering the binding properties of the epidermal growth factor receptor. This accounts for much of the differences between values reported for epidermal growth factor concentration in blood by investigators using different assay procedures.
人全血血清与70 - 100 ng/ml的小鼠表皮生长因子一样，能降低[125I]标记的小鼠表皮生长因子与培养的人成纤维细胞的结合。然而，在人全血血清中检测到的结合抑制剂中，与表皮生长因子相关的最多占1 - 2%，其余部分由血清制备过程中释放的其他因子组成，主要是血小板衍生生长因子，这些因子能够改变表皮生长因子受体的结合特性。这就解释了使用不同检测方法的研究人员所报告的血液中表皮生长因子浓度值之间的许多差异。
