DMRQC与欧加农联合进行的通过酶联免疫吸附测定法检测甲型肝炎IgM的现场试验报告。
Report of a joint DMRQC/Organon field trial to detect hepatitis A IgM by ELISA.
作者信息
Supran E M, Craske J, Hart R J, Kurtz J B, Parry J V, Skidmore S J, Gardner P S
出版信息
J Clin Pathol. 1983 Oct;36(10):1111-5. doi: 10.1136/jcp.36.10.1111.
The results of a field trial of a joint DMRQC/Organon ELISA kit for the detection of hepatitis A IgM antibody are reported. The participating laboratories were asked to use the kit to test a panel of 360 specimens consisting of duplicate coded samples of 180 sera. The panel was also tested by MACRIA in the Virus Reference Laboratory, Colindale. The ELISA was shown to be specific and sensitive giving good discrimination between acute and late convalescent hepatitis A sera. It was proposed that the same cut-off control as is used in the RIA (equivalent to 10 RIA units) should be adopted for the ELISA also.
本文报告了一种用于检测甲型肝炎IgM抗体的DMRQC/Organon联合酶联免疫吸附测定（ELISA）试剂盒的现场试验结果。参与试验的实验室被要求使用该试剂盒检测一组360份标本，其中包括180份血清的重复编码样本。该样本组也由科林代尔病毒参考实验室的MACRIA进行了检测。结果表明，该ELISA具有特异性和敏感性，能很好地区分急性和恢复期后期的甲型肝炎血清。建议ELISA也采用与放射免疫分析（RIA）相同的临界对照值（相当于10个RIA单位）。
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