Duermeyer W, Wielaard F, van der Veen J
J Med Virol. 1979;4(1):25-32. doi: 10.1002/jmv.1890040104.
A new test principle for the detection of specific IgM-class antibodies was developed and applied in an Enzyme-Linked Immuno Sorbent Assay (ELISA) for the detection of hepatitis A IgM antibodies. A solid phase coated with anti-IgM was incubated successively with serum sample, specific antigen, and enzyme-labeled F (ab')2 fragments from IgG antibodies against the antigen and enzyme substrate. F(ab')2 fragments were used to avoid interference with rheumatoid factor. Specificity and sensitivity are very high. This test principle appears generally applicable in the diagnosis of infectious and parasitic diseases by testing only one serum sample.
一种用于检测特异性IgM类抗体的新检测原理被研发出来，并应用于酶联免疫吸附测定（ELISA）中以检测甲型肝炎IgM抗体。将包被有抗IgM的固相载体依次与血清样本、特异性抗原、针对该抗原的IgG抗体的酶标记F(ab')2片段以及酶底物一起孵育。使用F(ab')2片段以避免类风湿因子的干扰。特异性和灵敏度都非常高。这一检测原理似乎普遍适用于仅通过检测一份血清样本诊断感染性疾病和寄生虫病。
