Frösner G G, Deinhardt F, Scheid R, Gauss-Müller V, Holmes N, Messelberger V, Siegl G, Alexander J J
Infection. 1979;7(6):303-5. doi: 10.1007/BF01642154.
Hepatitis A virus (HAV) was isolated directly from human feces and propagated serially in an HBsAg producing human hepatoma cell line. No cytopathic effect was observed in the tissue culture and no detectable amounts of HAV were present in the tissue culture supernatant fluid. However, increasing amounts of hepatitis A antigen (HAAg) were detected by radioimmunoassay in the cell extracts obtained by freezing and thawing of cells. Specificity of the HAAg determination was shown by neutralization with convalescent sera of marmosets experimentally infected with the MS-1 strain of hepatitis A and by the absence of this neutralization with preinoculation sera. HAAg was first detected after four weeks in the cell extract of infected cultures after inoculation of 10(2)--10(4) tissue culture infectious doses of HAV from second passage.
甲型肝炎病毒（HAV）直接从人类粪便中分离出来，并在一个产生乙肝表面抗原（HBsAg）的人肝癌细胞系中连续传代培养。在组织培养中未观察到细胞病变效应，且在组织培养上清液中未检测到可检测量的甲型肝炎病毒。然而，通过对经冻融处理的细胞提取物进行放射免疫测定，检测到细胞提取物中的甲型肝炎抗原（HAAg）量不断增加。用实验感染甲型肝炎MS-1株的狨猴恢复期血清进行中和试验，以及用接种前血清进行中和试验均未出现中和反应，这表明了HAAg测定的特异性。在接种来自第二代传代的10(2)-10(4)个组织培养感染剂量的甲型肝炎病毒后四周，首次在受感染培养物的细胞提取物中检测到HAAg。
