Cassan M, Ronceray J, Patte J C
Nucleic Acids Res. 1983 Sep 24;11(18):6157-66. doi: 10.1093/nar/11.18.6157.
The regulatory region of the lysC gene (that encodes the lysine-sensitive aspartokinase of Escherichia coli) has been identified and purified by the use of lysC-lacZ fusions. Its regulatory sequence has been determined. No signals similar to those described in the case of an attenuation mechanism could be found in the long leader sequence existing between the starts of transcription and of translation.
通过使用lysC-lacZ融合技术,已鉴定并纯化了lysC基因(编码大肠杆菌赖氨酸敏感天冬氨酸激酶)的调控区域。其调控序列已被确定。在转录起始和翻译起始之间存在的长前导序列中,未发现与衰减机制中所描述的信号类似的信号。