从人扁桃体中克隆白细胞介素2信使核糖核酸

Cloning of interleukin 2 mRNAs from human tonsils.

作者信息

Maeda S, Nishino N, Obaru K, Mita S, Nomiyama H, Shimada K, Fujimoto K, Teranishi T, Hirano T, Onoue K

出版信息

Biochem Biophys Res Commun. 1983 Sep 30;115(3):1040-7. doi: 10.1016/s0006-291x(83)80040-0.

Abstract

Human interleukin 2 (IL-2) mRNAs were cloned from a cDNA library prepared from mitogen-stimulated tonsillar mononuclear cells. One of these clones was sequenced and, using this cDNA as a hybridization probe, Southern blot analysis of the human placental DNA was performed. Our results indicate that there is only one IL-2 gene in the human genome.

摘要

人白细胞介素2（IL-2）信使核糖核酸是从经丝裂原刺激的扁桃体单核细胞制备的cDNA文库中克隆出来的。对其中一个克隆进行了测序，并以该cDNA作为杂交探针，对人胎盘DNA进行了Southern印迹分析。我们的结果表明，人类基因组中只有一个IL-2基因。