Heidmann O, Rougeon F
Cell. 1983 Oct;34(3):767-77. doi: 10.1016/0092-8674(83)90533-0.
To analyze the rabbit immunoglobulin kappa locus, we isolated, from a phenotypically homozygous b4/b4 rabbit genomic library, a C kappa gene corresponding to the b4 var allelic form of the b4 C kappa gene. We also determined the nucleotide sequence of the J kappa cluster 3 kb upstream of the b4 var C kappa gene. Southern blot experiments with rabbit J kappa probes indicate that the bas C kappa gene is an isotype probably associated with its own J kappa segment(s). The rabbit b4 J kappa gene region contains a cluster of five J kappa segments, homologous to the human J kappa cluster, and two remnant J kappa segments approximately 5 kb upstream of the b4 C kappa gene. Nucleotide sequence analyses of the coding J kappa segments and their recombination signal sequences show only one functional J kappa segment. Comparisons with the protein data indicate that the rabbit kappa gene family, unlike its human and mouse homologs, increases kappa light chain variability by deletions and insertions at the V-J junction.
为了分析兔免疫球蛋白κ基因座，我们从表型纯合的b4/b4兔基因组文库中分离出一个与b4 Cκ基因的b4 var等位基因形式相对应的Cκ基因。我们还测定了b4 var Cκ基因上游3 kb处Jκ簇的核苷酸序列。用兔Jκ探针进行的Southern印迹实验表明，bas Cκ基因是一种同型，可能与其自身的Jκ片段相关。兔b4 Jκ基因区域包含一组五个与人类Jκ簇同源的Jκ片段，以及在b4 Cκ基因上游约5 kb处的两个残余Jκ片段。对编码Jκ片段及其重组信号序列的核苷酸序列分析表明只有一个功能性Jκ片段。与蛋白质数据的比较表明，兔κ基因家族与其人类和小鼠同源物不同，通过在V-J连接处的缺失和插入增加κ轻链的变异性。
