Emorine L, Dreher K, Kindt T J, Max E E
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5709-13. doi: 10.1073/pnas.80.18.5709.
To investigate the genetic mechanism by which certain rabbits can express immunoglobulins unexpected on the basis of their pedigree (i.e., "latent" allotypes), we have begun a study of the rabbit immunoglobulin kappa gene locus. Here we report the structure of a germline genomic clone that encodes the b4 allotype of rabbit kappa immunoglobulin and corresponds to the kappa gene expressed by the rabbit-mouse hybridoma 12F2. The nucleotide sequences of the joining (J) and constant (C) regions reveal structures generally similar to the homologous mouse and human loci, although only one of the five J-like sequences of this rabbit gene is apparently expressed. Southern blotting analysis of DNA from several rabbit allotypic strains by using probes derived from the cloned b4 gene demonstrates that, in contrast to mouse and human, rabbits possess multiple kappa-related sequences. Rabbits of the nominal b4, b5, b6, and b9 allotypes each contain at least two b4-related sequences that are associated with their own J regions and that are highly homologous to the cloned b4 gene in both coding and flanking regions.
为了研究某些兔子能够表达基于其谱系预期之外的免疫球蛋白（即“潜在”同种异型）的遗传机制，我们开始了对兔子免疫球蛋白κ基因座的研究。在此，我们报道了一个种系基因组克隆的结构，该克隆编码兔子κ免疫球蛋白的b4同种异型，并且与兔 - 鼠杂交瘤12F2所表达的κ基因相对应。连接（J）区和恒定（C）区的核苷酸序列揭示了与同源的小鼠和人类基因座总体相似的结构，尽管该兔子基因的五个类J序列中只有一个明显表达。使用来自克隆的b4基因的探针，对几种兔子同种异型品系的DNA进行Southern印迹分析表明，与小鼠和人类不同，兔子拥有多个κ相关序列。名义上为b4、b5、b6和b9同种异型的兔子至少拥有两个与它们自己的J区相关的b4相关序列，并且在编码区和侧翼区与克隆的b4基因高度同源。
