Singhal R P, Griffin G D, Novelli G D
Biochemistry. 1976 Nov 16;15(23):5083-7. doi: 10.1021/bi00668a021.
The transfer RNA separation by chromatography on strong-base polystyrene exchange materials is examined and compared with the widely used reversed-phase chromatography. Results indicate important differences in some transfer RNA (tRNA) elution patterns by the anion-exchange chromatography, as compared with the reversed-phase chromatography. Transfer RNAs containing hydrophobic groups are adsorbed more strongly. The anion exchanger has twice the number of theoretical plates. Single peaks of tRNA2Glu and tRNA1Phe obtained from the reversed-phase column give multiple peaks only polystyrene anion-exchange chromatography. All six leucine tRNAs (Escherichia coli) and differences in tRNA populations synthesized during early and late stages of the dividing lymphocytes from normal human blood can be characterized by the anion-exchange chromatography. Different separation profiles are obtained by two separation systems for tyrosine tRNAs from mouse liver and mouse-plasma-cell tumor. The results indicate that, in contrast to the reversed-phase chromatography, strong-base-polystyrene anion-exchange chromatography is capable of separating tRNAs with minor structlral differences.
研究了通过强碱性聚苯乙烯交换材料上的色谱法分离转运RNA，并与广泛使用的反相色谱法进行了比较。结果表明，与反相色谱法相比，阴离子交换色谱法在某些转运RNA（tRNA）洗脱模式上存在重要差异。含有疏水基团的转运RNA吸附更强。阴离子交换剂的理论塔板数是其两倍。从反相柱获得的tRNA2Glu和tRNA1Phe的单峰在聚苯乙烯阴离子交换色谱中仅产生多个峰。所有六种亮氨酸tRNA（大肠杆菌）以及正常人血液中分裂淋巴细胞早期和晚期合成的tRNA群体差异都可以通过阴离子交换色谱法进行表征。从小鼠肝脏和小鼠浆细胞瘤中分离酪氨酸tRNA的两种分离系统获得了不同的分离图谱。结果表明，与反相色谱法不同，强碱性聚苯乙烯阴离子交换色谱法能够分离结构差异较小的tRNA。
