磷酸蔗糖和山梨醇对包膜病毒储存期间感染性的影响。
Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage.
作者信息
Howell C L, Miller M J
出版信息
J Clin Microbiol. 1983 Sep;18(3):658-62. doi: 10.1128/jcm.18.3.658-662.1983.
Abstract
Cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 M SP) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees C over time. Recovery from 0.2 M SP was uniformly better. More tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 M SP virus stocks as compared with viruses stored in 70% sorbitol. Although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing diluted virus suspensions generally resulted in diminished recovery. Similar stabilizing effects on respiratory syncytial virus and herpes simplex virus type 1 infectivities were observed when stocks were preserved in 0.2 M SP, as compared with 70% sorbitol. Overall, 0.2 M SP was better than 70% sorbitol for stabilizing cytomegalovirus, varicella-zoster virus, respiratory syncytial virus, and herpes simplex virus type 1 infectivities under the conditions tested.
摘要
比较了巨细胞病毒和水痘带状疱疹病毒在-70℃、4℃和20℃下储存一段时间后从磷酸蔗糖（0.2M SP）和70%山梨醇（山梨醇）中的回收率。从0.2M SP中的回收率始终更高。与储存在70%山梨醇中的病毒相比，接种0.2M SP病毒储备液的细胞单层中回收的组织培养感染剂量和感染灶更多。尽管两种病毒均从稀释的新鲜储备液（10^-1至10^-4）中分离出来，但冷冻稀释的病毒悬液通常会导致回收率降低。与70%山梨醇相比，当储备液保存在0.2M SP中时，对呼吸道合胞病毒和1型单纯疱疹病毒的感染性也观察到了类似的稳定作用。总体而言，在测试条件下，0.2M SP在稳定巨细胞病毒、水痘带状疱疹病毒、呼吸道合胞病毒和1型单纯疱疹病毒的感染性方面优于70%山梨醇。
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