零下温度下细胞水分流失的动力学及细胞内结冰的可能性
KINETICS OF WATER LOSS FROM CELLS AT SUBZERO TEMPERATURES AND THE LIKELIHOOD OF INTRACELLULAR FREEZING.
作者信息
MAZUR P
出版信息
J Gen Physiol. 1963 Nov;47(2):347-69. doi: 10.1085/jgp.47.2.347.
Abstract
The survival of various cells subjected to low temperature exposure is higher when they are cooled slowly. This increase is consistent with the view that slow cooling decreases the probability of intracellular freezing by permitting water to leave the cell rapidly enough to keep the protoplasm at its freezing point. The present study derives a quantitative relation between the amount of water in a cell and temperature. The relation is a differential equation involving cooling rate, surface-volume ratio, membrane permeability to water, and the temperature coefficient of the permeability constant. Numerical solutions to this equation give calculated water contents which permit predictions as to the likelihood of intracellular ice formation. Both the calculated water contents and the predictions on internal freezing are consistent with the experimental observations of several investigators.
摘要
当各种细胞缓慢冷却时，它们在低温暴露下的存活率更高。这种增加与以下观点一致，即缓慢冷却通过允许水足够快地离开细胞，以保持原生质处于其冰点，从而降低了细胞内结冰的可能性。本研究推导了细胞内水量与温度之间的定量关系。该关系是一个微分方程，涉及冷却速率、表面积与体积比、膜对水的渗透性以及渗透常数的温度系数。该方程的数值解给出了计算出的含水量，这些含水量可以预测细胞内结冰的可能性。计算出的含水量以及关于内部结冰的预测都与几位研究人员的实验观察结果一致。
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