Silver-Mysliwiec T H, Bramucci M G
Department of Microbiology and Immunology, Hahnemann University, Philadelphia, Pennsylvania 19102.
J Bacteriol. 1990 Apr;172(4):1948-53. doi: 10.1128/jb.172.4.1948-1953.1990.
The previously characterized bacteriophage SP10 enhanced the frequency of wild-type sporulation by Bacillus subtilis W23 and 3-13. Comparison of SP10 with the spore-converting bacteriophage PMB12 indicated that both bacteriophages significantly increased the sporulation frequency of an oligosporogenic mutant that contained spo0J::Tn917 omega HU261. SP10 and PMB12 caused wild-type bacteria to sporulate in a liquid medium that initially contained enough glucose to inhibit the sporulation and expression of alpha-amylase by uninfected bacteria. SP10 also induced the expression of alpha-amylase in the presence of glucose, whereas PMB12 had no detectable effect. These observations were consistent with the conclusion that SP10 is a spore-converting bacteriophage and that SP10 and PMB12 relieve glucose-mediated catabolite repression of sporulation by different mechanisms.
先前鉴定的噬菌体SP10提高了枯草芽孢杆菌W23和3 - 13野生型孢子形成的频率。将SP10与孢子转化噬菌体PMB12进行比较表明，这两种噬菌体均显著提高了含有spo0J::Tn917 ω HU261的寡孢子形成突变体的孢子形成频率。SP10和PMB12能使野生型细菌在最初含有足够葡萄糖以抑制未感染细菌孢子形成和α -淀粉酶表达的液体培养基中形成孢子。SP10还能在有葡萄糖存在的情况下诱导α -淀粉酶的表达，而PMB12则没有可检测到的影响。这些观察结果与以下结论一致：SP10是一种孢子转化噬菌体，并且SP10和PMB12通过不同机制缓解了葡萄糖介导的孢子形成的分解代谢阻遏。
