Tomlinson A H, Esiri M M
J Neurol Sci. 1983 Aug-Sep;60(3):473-84. doi: 10.1016/0022-510x(83)90158-2.
Six-week-old Balb/c mice were inoculated intranasally with a suspension of HSV1 virus and the distribution of viral antigen in the brain 3-7 days later was surveyed using the immunoperoxidase technique. Virus was first detectable in the brain 4 days later at 2 distinct sites: the trigeminal root entry zone in the brain stem and the olfactory bulbs. On succeeding days virus spread from the trigeminal focus to many other brain stem nuclei and, in some mice, to the thalamus and the cerebellum. From the olfactory bulbs, in a proportion of mice, virus spread to anterior olfactory nucleus, lateral olfactory tract, septal nuclei, temporal lobe, hippocampus and cingulate cortex. Infection of olfactory bulbs was found to occur following intracorneal as well as intranasal inoculation of virus. The relevance of this model to human herpes simplex encephalitis is discussed.
六周龄的Balb/c小鼠经鼻内接种单纯疱疹病毒1型悬液，3至7天后采用免疫过氧化物酶技术检测病毒抗原在脑内的分布。4天后在脑内两个不同部位首次检测到病毒：脑干的三叉神经根进入区和嗅球。在随后的几天里，病毒从三叉神经病灶扩散到许多其他脑干核团，在一些小鼠中还扩散到丘脑和小脑。在一部分小鼠中，病毒从嗅球扩散到前嗅核、外侧嗅束、隔核、颞叶、海马和扣带回皮质。发现角膜内和鼻内接种病毒后均会发生嗅球感染。讨论了该模型与人类单纯疱疹性脑炎的相关性。
