Espion D, Kaiser K, Dambly-Chaudiere C
J Mol Biol. 1983 Nov 5;170(3):611-33. doi: 10.1016/s0022-2836(83)80124-7.
We describe the isolation and characterization of a new Q-independent substitution mutant of lambda, lambdaqin111, which differs from other characterized Q-independent lambda phages. This mutant defines a new lambda-like prophage in the bacterial chromosome, as seen by homologous recombination between lambdaqin111 and the host DNA and by DNA/DNA hybridization methods. Genetic and electron microscopy data show that this new prophage carries, at least, genes analogous to Q-S-R of lambda and also a cos site functionally identical to lambda cos. It is located near 34 min on the Escherichia coli K12 map, i.e. in the same region but at a different site from the defective Rac prophage.
我们描述了一种新的λ噬菌体的Q非依赖型替代突变体λqin111的分离和特性，它与其他已鉴定的Q非依赖型λ噬菌体不同。通过λqin111与宿主DNA之间的同源重组以及DNA/DNA杂交方法可以看出，该突变体在细菌染色体中定义了一种新的类λ原噬菌体。遗传和电子显微镜数据表明，这种新的原噬菌体至少携带与λ的Q-S-R类似的基因，以及一个功能上与λcos相同的粘性末端位点。它位于大肠杆菌K12图谱上34分钟附近，即在同一区域，但与缺陷型Rac原噬菌体处于不同位点。
