Dore E, Frontali C, Forte T, Fratarcangeli S
Mol Biochem Parasitol. 1983 Aug;8(4):339-52. doi: 10.1016/0166-6851(83)90080-4.
The average length and the interspersion pattern of repetitive DNA sequences in the Plasmodium berghei genome have been studied by electron microscopy. Within the limitations posed by the relatively high genome complexity, analysis of partially renatured total DNA indicates that repetitive sequences do not occupy preferential positions along the genome, but are widely dispersed (one in approx. 8000 base pairs of unique DNA). Structures appearing as loops flanked by inverted repeats are present. Analysis of the repetitive fraction purified by hydroxyapatite chromatography shows that the average length of rapidly reassociating repetitive structures is around 800 base pairs with 90% of the length distribution between 400 and 1400 base pairs. Suitable extraction methods, preserving circularity of extrachromosomal DNA components, allow the detection of molecules which can be identified as mitochondrial DNA, 10.5 +/- 0.4 microns long.
通过电子显微镜研究了伯氏疟原虫基因组中重复DNA序列的平均长度和散布模式。在相对较高的基因组复杂性所带来的限制范围内，对部分复性的总DNA的分析表明，重复序列在基因组中并不占据优先位置，而是广泛散布（大约每8000个碱基对的独特DNA中有一个）。存在由反向重复序列侧翼的环状结构。对通过羟基磷灰石柱色谱法纯化的重复部分的分析表明，快速重新缔合的重复结构的平均长度约为800个碱基对，90%的长度分布在400至1400个碱基对之间。合适的提取方法可保留染色体外DNA成分的环状结构，从而能够检测到可被鉴定为线粒体DNA的分子，其长度为10.5±0.4微米。
