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从禽疟原虫鸡疟原虫中分离出的一种保守的染色体外元件的特性分析。

Characterization of a conserved extrachromosomal element isolated from the avian malarial parasite Plasmodium gallinaceum.

作者信息

Joseph J T, Aldritt S M, Unnasch T, Puijalon O, Wirth D F

机构信息

Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts.

出版信息

Mol Cell Biol. 1989 Sep;9(9):3621-9. doi: 10.1128/mcb.9.9.3621-3629.1989.

DOI:10.1128/mcb.9.9.3621-3629.1989
PMID:2779561
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362422/
Abstract

We have identified a conserved, repeated, and highly transcribed DNA element from the avian malarial parasite Plasmodium gallinaceum. The element produced multiple transcripts in both zygotes and asexual blood stages of this parasite. It was found to be highly conserved in all of five malarial species tested and hybridized at reduced stringency to other members of the phylum Apicomplexa, including the genera Babesia, Eimeria, Toxoplasma, and Theileria. The copy number of the element was about 15, and it had a circularly permuted restriction map with a repeat unit length of about 6.2 kilobases. It could be separated from the main genomic DNA by using sucrose gradients and agarose gels, and it migrated separately from the recognized Plasmodium chromosomes on pulse-field gels. In the accompanying paper (S. M. Aldritt, J. T. Joseph, and D. F. Wirth, Mol. Cell. Biol. 9:3614-3620, 1989), evidence is presented that element contains the mitochondrial genes for the protein cytochrome b and a fragment of the large rRNA. We postulate that this element is an episome in the mitochondria of the obligate parasites belonging to the phylum Apicomplexa.

摘要

我们已从鸡疟原虫（Plasmodium gallinaceum）中鉴定出一种保守、重复且转录高度活跃的DNA元件。该元件在这种寄生虫的合子和无性血液阶段均产生多种转录本。在所有测试的五种疟原虫物种中，它都高度保守，并能以较低的严谨性与顶复门（Apicomplexa）的其他成员杂交，包括巴贝斯虫属（Babesia）、艾美耳球虫属（Eimeria）、弓形虫属（Toxoplasma）和泰勒虫属（Theileria）。该元件的拷贝数约为15，其限制性酶切图谱呈环状排列，重复单元长度约为6.2千碱基对。通过使用蔗糖梯度和琼脂糖凝胶，它可与主要基因组DNA分离，并且在脉冲场凝胶上，它与公认的疟原虫染色体迁移轨迹不同。在随附的论文（S. M. Aldritt、J. T. Joseph和D. F. Wirth，《分子细胞生物学》9:3614 - 3620，1989年）中，有证据表明该元件包含细胞色素b蛋白的线粒体基因和大核糖体RNA的一个片段。我们推测该元件是顶复门专性寄生虫线粒体中的一种附加体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/4ab1a2a1029d/molcellb00057-0047-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/a720d1f7b717/molcellb00057-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/960e5783990e/molcellb00057-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/f80e3b73337e/molcellb00057-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/6735b3ff0298/molcellb00057-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/a05f0975c85c/molcellb00057-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/7c69fff12ced/molcellb00057-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/288d1ede3134/molcellb00057-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/4ab1a2a1029d/molcellb00057-0047-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/a720d1f7b717/molcellb00057-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/960e5783990e/molcellb00057-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/f80e3b73337e/molcellb00057-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/6735b3ff0298/molcellb00057-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/a05f0975c85c/molcellb00057-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/7c69fff12ced/molcellb00057-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/288d1ede3134/molcellb00057-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd1/362422/4ab1a2a1029d/molcellb00057-0047-b.jpg

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