Hamill O P, Bormann J, Sakmann B
Nature. 1983;305(5937):805-8. doi: 10.1038/305805a0.
In the mammalian central nervous system, glycine and gamma-aminobutyric acid (GABA) bind to specific and distinct receptors and cause an increase in membrane conductance to CI- (refs 5-7). Neurones in various regions of the nervous system show differential sensitivity to glycine and GABA; thus GABA and glycine receptors are spatially distinct from one another. However, on the basis of desensitization experiments on spinal cord neurones, it was suggested that the receptors for glycine and GABA may share the same CI- channel. We now report that in small membrane patches, isolated from the soma of spinal neurones, both receptor channels display several (multiple) conductance states. Two of the states are common to both receptor channels. However, the most frequently observed 'main conductance states' of the GABA and glycine receptor channels are different. Both channels display the same anion selectivity. We propose that one class of multistate CI- channel is coupled to either GABA or glycine receptors. The main conductance state adopted by this channel is determined by the receptor to which it is coupled.
在哺乳动物的中枢神经系统中，甘氨酸和γ-氨基丁酸（GABA）与特定且不同的受体结合，并导致氯离子（Cl⁻）的膜电导增加（参考文献5 - 7）。神经系统不同区域的神经元对甘氨酸和GABA表现出不同的敏感性；因此，GABA和甘氨酸受体在空间上彼此不同。然而，基于对脊髓神经元的脱敏实验，有人提出甘氨酸和GABA的受体可能共用同一个Cl⁻通道。我们现在报告，从脊髓神经元胞体分离出的小膜片中，两种受体通道都表现出几种（多个）电导状态。其中两种状态是两种受体通道共有的。然而，GABA和甘氨酸受体通道最常观察到的“主导电状态”是不同的。两种通道表现出相同的阴离子选择性。我们提出，一类多状态Cl⁻通道与GABA或甘氨酸受体之一偶联。该通道采用的主导电状态由与其偶联的受体决定。
