Tralka T S, Flatow U, Goldstein S, Rabson A S
Proc Soc Exp Biol Med. 1983 Oct;174(1):41-6. doi: 10.3181/00379727-174-41701.
A cell line derived from hamster spleen transformed by human papovavirus BK was adapted to grow in 5 mM butyrate. Ultrastructurally, the butyrate-adapted cells were found to contain large numbers of type-R and intracytoplasmic type-A virus-like particles (VLP). In contrast, the unadapted cells contained only rare VLP. Treatment of the unadapted cells with nontoxic and toxic concentrations of butyrate for 24 and 48 hr failed to induce VLP, indicating the importance of butyrate adaptation in the induction of VLP. The increase in type-R VLP was stable after removal of the adapted cells from butyrate; however, the increase in type-A VLP was not.
一株由人乳头多瘤病毒BK转化的仓鼠脾脏衍生细胞系经适应后可在5 mM丁酸盐中生长。超微结构上，适应丁酸盐的细胞含有大量R型和胞质A型病毒样颗粒（VLP）。相比之下，未适应的细胞仅含有罕见的VLP。用无毒和有毒浓度的丁酸盐处理未适应的细胞24小时和48小时未能诱导VLP，这表明丁酸盐适应在诱导VLP中的重要性。从丁酸盐中去除适应细胞后，R型VLP的增加是稳定的；然而，A型VLP的增加则不然。
