人乳头多瘤空泡病毒BK基因组在转化仓鼠细胞中的一致表现
Uniform representation of the human papovavirus BK genome in transformed hamster cells.
作者信息
Howley P M, Martin M A
出版信息
J Virol. 1977 Jul;23(1):205-8. doi: 10.1128/JVI.23.1.205-208.1977.
Abstract
The DNA of three cloned lines of hamster kidney cells transformed by human papovavirus BK DNA was examined by reassociation kinetics for viral sequences and found to contain 2.7 to 5.3 equivalents of viral DNA per diploid genome. In the one line examined with the four R-HindIII fragments of the human papovavirus BK genome, the entire viral genome was uniformly represented.
摘要
通过重缔合动力学对人乳头多瘤病毒BK DNA转化的三个仓鼠肾细胞克隆系的DNA进行病毒序列检测，发现每个二倍体基因组含有2.7至5.3个病毒DNA当量。在用人类乳头多瘤病毒BK基因组的四个R-HindIII片段检测的一个细胞系中，整个病毒基因组均一表达。
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