Hara K, Ito M, Takeuchi J, Iijima S, Endo T, Hidaka H
Virchows Arch A Pathol Anat Histopathol. 1983;401(2):237-49. doi: 10.1007/BF00692648.
Immunohistochemical studies were performed for the presence of S-100b protein in non-neoplastic and neoplastic salivary gland tissues by the peroxidase anti-peroxidase (PAP) method. Some cases of pleomorphic adenoma were investigated by immuno-electron microscopy. S-100b protein could not be detected in epithelial cells of intercalated ducts, acini, striated ducts and excretory ducts of non-neoplastic salivary gland. However, myoepithelial cells surrounding the acini and intercalated ducts were specifically stained by S-100b protein. In pleomorphic adenomas, S-100b protein-positive cells could be mostly observed in the myxoid and chondroid areas, and the basal layer cells of the double-layered ductal cells were also positive. In clear cell adenoma, the clear cells were also S-100b protein positive. In adenoid cystic carcinomas, S-100b protein-positive cells could be found in trabecular areas, but not in tumor cells showing cribriform-pattern. In other tumors (Warthin's tumor, oxyphilic adenoma, basal cell adenoma, mucoepidermoid tumor and acinar cell carcinoma), S-100b protein positive cells were seldom observed. Immuno-electron microscopically, S-100b protein was diffusely distributed in the cytoplasm of myoepithelial cells as well as of tumor cells of pleomorphic adenoma, being distributed especially on the membrane of endoplasmic reticulum and the outer nuclear membrane.
采用过氧化物酶抗过氧化物酶（PAP）法对非肿瘤性和肿瘤性涎腺组织中S-100b蛋白的存在情况进行免疫组织化学研究。对部分多形性腺瘤病例进行了免疫电子显微镜检查。在非肿瘤性涎腺的闰管、腺泡、纹状管和排泄管的上皮细胞中未检测到S-100b蛋白。然而，腺泡和闰管周围的肌上皮细胞被S-100b蛋白特异性染色。在多形性腺瘤中，S-100b蛋白阳性细胞主要见于黏液样和软骨样区域，双层导管细胞的基底层细胞也呈阳性。在透明细胞腺瘤中，透明细胞也呈S-100b蛋白阳性。在腺样囊性癌中，S-100b蛋白阳性细胞可见于小梁区域，但筛状结构的肿瘤细胞中未见。在其他肿瘤（沃辛瘤、嗜酸性腺瘤、基底细胞腺瘤、黏液表皮样癌和腺泡细胞癌）中，很少观察到S-100b蛋白阳性细胞。免疫电子显微镜下，S-100b蛋白弥漫分布于多形性腺瘤肌上皮细胞和肿瘤细胞的细胞质中，尤其分布在内质网膜和外核膜上。
