Gormley I P, Bolton R E, Brown G M, Davis J M, Wright A
Environ Health Perspect. 1983 Sep;51:35-9. doi: 10.1289/ehp.835135.
Samples of the chrysotile taken during and after treatment by the wet dispersion process have been tested for their cytotoxic effect in vitro and the results compared with both a UICC chrysotile A sample and a dust prepared from a standard chrysotile textile yarn. Results were obtained from three different in vitro assay systems utilizing P388D1, V79-4 and A549 cells. A sample which still contained the wetting agent used in the wet dispersion process failed to show activity in any of these assays. The other samples, however, were all active with those dusts obtained by milling the final product and by sampling the air of the factory consistently proving significantly more cytotoxic than the standard chrysotile controls. Preliminary results from a parallel in vivo study suggest that these samples are also more active in producing mesotheliomas in rats.
对温法分散处理过程中及处理后的温石棉样品进行了体外细胞毒性作用测试，并将结果与国际癌症研究机构（UICC）的温石棉A样品以及由标准温石棉纺织纱线制备的粉尘进行了比较。结果来自利用P388D1、V79 - 4和A549细胞的三种不同体外检测系统。一个仍含有温法分散过程中使用的湿润剂的样品在任何这些检测中均未显示出活性。然而，其他样品均具有活性，通过研磨最终产品获得的粉尘以及在工厂空气中采样得到的粉尘始终被证明比标准温石棉对照具有明显更强的细胞毒性。一项平行体内研究的初步结果表明，这些样品在大鼠中诱发间皮瘤方面也更具活性。
