Thamann T J, Lord R C, Wang A H, Rich A
Nucleic Acids Res. 1981 Oct 24;9(20):5443-57. doi: 10.1093/nar/9.20.5443.
The laser-Raman spectra of crystalline d(CpGpCpGpCpG) and of aqueous poly(dG-dC).poly(dG-dC) in high salt (4M NaCl) and low salt (0.1M NaCl) solutions have been measured and compared. The spectra of the crystal and the high-salt solution show a striking congruence, which indicates clearly that the high-salt form of the aqueous polymer has the left-handed Z-DNA structure of the crystalline oligomer. These two spectra differ substantially from that of the low-salt form of the polymer, which has been found previously to have spectral characteristics of the B-form of DNA. The high salt spectrum shows a unique line due to guanine residues at 625 cm-1 which should be useful for qualitative and possibly quantitative assessment of the amount of Z-structure present in a sample of DNA.
已对结晶态的d（CpGpCpGpCpG）以及高盐（4M NaCl）和低盐（0.1M NaCl）溶液中的水性聚（dG-dC）.聚（dG-dC）的激光拉曼光谱进行了测量和比较。晶体和高盐溶液的光谱显示出惊人的一致性，这清楚地表明水性聚合物的高盐形式具有结晶寡聚物的左手Z-DNA结构。这两种光谱与聚合物的低盐形式的光谱有很大不同，先前已发现低盐形式具有DNA B型的光谱特征。高盐光谱在625 cm-1处显示出一条由于鸟嘌呤残基产生的独特谱线，这对于定性以及可能定量评估DNA样品中存在的Z结构量应该是有用的。
