Cranage M P, Gurner B W, Coombs R R
J Immunol Methods. 1983 Nov 11;64(1-2):7-16. doi: 10.1016/0022-1759(83)90378-2.
A simple method for stabilising antibody-linked red blood cells by the addition of low concentrations of glutaraldehyde is described. Fresh and stabilised reagent-linked cells were shown to compare favourably in reverse passive haemagglutination for the measurement of human immunoglobulin isotypes, G, A and M and for the detection of respiratory syncytial and herpes simplex viruses. Stabilised cells were also used to detect antibodies to bacteria and to a soluble antigen adsorbed to a solid phase by mixed haemagglutination reactions.
本文描述了一种通过添加低浓度戊二醛来稳定抗体连接红细胞的简单方法。在用于检测人免疫球蛋白G、A和M同种型以及呼吸道合胞病毒和单纯疱疹病毒的反向被动血凝试验中，新鲜的和经稳定处理的试剂连接细胞表现出良好的效果。稳定化细胞还被用于通过混合血凝反应检测针对细菌的抗体以及吸附在固相上的可溶性抗原。
