Grossmann A, Eggers-Schumacher G, Sander E
Biologisches Institut, Universität Tübingen, Germany.
J Immunol Methods. 1995 Feb 27;179(2):243-50. doi: 10.1016/0022-1759(94)00291-4.
Methods are described for the detection of potato virus X (PVX) by reverse passive haemagglutination (RPH) by means of polyclonal antiviral antibodies coupled to sheep red blood cells (sRBC) and the chromic chloride method. The cells were stabilized with pyruvic aldehyde, thus providing a stock suspension for numerous coupling experiments lasting several months. Anti-PVX IgY, which is readily isolated in large amounts from the egg yolk of immunized chickens, was used in an avidin-biotin enhanced RPH assay with stabilized sRBC. With this method the PVX detection rate achieved was comparable to that of RPH assays using fresh non-fixed sRBC. In addition, avidin-coated sRBC could be stored for weeks at 4 degrees C and subsequently used for coupling with biotinylated IgY.
本文描述了通过反向被动血凝反应（RPH）检测马铃薯X病毒（PVX）的方法，该方法借助与绵羊红细胞（sRBC）偶联的多克隆抗病毒抗体以及氯化铬法。细胞用丙酮酸醛进行稳定处理，从而提供了用于持续数月的大量偶联实验的储备悬浮液。抗PVX IgY易于从免疫鸡的蛋黄中大量分离出来，在使用稳定化sRBC的抗生物素蛋白-生物素增强RPH检测中使用。用这种方法获得的PVX检测率与使用新鲜未固定sRBC的RPH检测相当。此外，抗生物素蛋白包被的sRBC可以在4℃下储存数周，随后用于与生物素化的IgY偶联。
