三种用于检测犊牛轮状病毒感染的快速诊断方法的比较
A comparison of three rapid diagnostic methods for the detection of rotavirus infection in calves.
作者信息
Edwards S, Chasey D, Napthine P, Banks J, Hewitt-Taylor C, Cranage M P
出版信息
Vet Microbiol. 1987 Jan;13(1):19-25. doi: 10.1016/0378-1135(87)90094-0.
Three techniques for the detection of rotavirus in faecal samples from calves with neonatal gastroenteritis were compared. A preliminary study indicated that reverse passive haemagglutination (RPHA) was at least as sensitive as the enzyme-linked immunosorbent assay (ELISA). These two immunoassays were compared with the detection of viral RNA by polyacrylamide gel electrophoresis (PAGE) on 209 field samples. Of the 77 samples in which at least one test gave a positive result, 69 were positive by both RPHA and PAGE, but only 49 were also positive by ELISA, indicating a lower sensitivity for the latter test. The overall agreement between RPHA and PAGE was 96%. The reasons for the discrepancies between the tests are discussed.
对三种检测新生犊牛胃肠炎粪便样本中轮状病毒的技术进行了比较。一项初步研究表明，反向被动血凝试验（RPHA）的敏感性至少与酶联免疫吸附测定（ELISA）相同。将这两种免疫测定法与通过聚丙烯酰胺凝胶电泳（PAGE）检测209份现场样本中的病毒RNA进行了比较。在至少一项检测呈阳性的77份样本中，69份通过RPHA和PAGE均呈阳性，但通过ELISA也呈阳性的仅49份，这表明后者检测的敏感性较低。RPHA和PAGE之间的总体一致性为96%。讨论了检测结果差异的原因。
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