Jans D A, Fimmel A L, Langman L, James L B, Downie J A, Senior A E, Ash G R, Gibson F, Cox G B
Biochem J. 1983 Jun 1;211(3):717-26. doi: 10.1042/bj2110717.
The amino acid substitutions in the mutant c-subunits of Escherichia coli F1F0-ATPase coded for by the uncE429, uncE408 and uncE463 alleles affect the incorporation of these proteins into the cell membrane. The DNA sequence of the uncE429 allele differed from normal in that a G leads to A base change occurred at nucleotide 68 of the uncE gene, resulting in glycine being replaced by aspartic acid at position 23 in the c-subunit. The uncE408 and uncE463 mutant DNA sequences were identical and differed from normal in that a C leads to T base change occurred at nucleotide 91 of the uncE gene, resulting in leucine being replaced by phenylalanine at position 31 in the c-subunit. An increased gene dosage of the uncE408 or uncE463 alleles resulted in the incorporation into the membranes of the mutant c-subunits. The results are discussed in terms of the 'Helical Hairpin Hypothesis' of Engelman & Steitz [(1981) Cell 23,411-422].
由uncE429、uncE408和uncE463等位基因编码的大肠杆菌F1F0 - ATP酶突变体c亚基中的氨基酸替换影响这些蛋白质整合到细胞膜中。uncE429等位基因的DNA序列与正常序列不同，在于uncE基因的第68位核苷酸发生了G到A的碱基变化，导致c亚基中第23位的甘氨酸被天冬氨酸取代。uncE408和uncE463突变体的DNA序列相同，与正常序列不同之处在于uncE基因的第91位核苷酸发生了C到T的碱基变化，导致c亚基中第31位的亮氨酸被苯丙氨酸取代。uncE408或uncE463等位基因剂量的增加导致突变体c亚基整合到膜中。根据Engelman和Steitz的“螺旋发夹假说”[(1981)细胞23,411 - 422]对结果进行了讨论。
