Law M F, Byrne J C, Howley P M
Mol Cell Biol. 1983 Nov;3(11):2110-5. doi: 10.1128/mcb.3.11.2110-2115.1983.
We describe a bovine papillomavirus hybrid plasmid containing the neomycin resistance gene from Tn5 inserted into a mammalian cell transcriptional unit. This plasmid is maintained as a stable extrachromosomal element (20 to 100 copies per diploid genome) in mouse cells selected either for the transformed phenotype or for resistance to the aminoglycoside G418. Cells selected for G418 resistance initially display a flat, nontransformed phenotype before exhibiting the gross morphological characteristics of transformation. The delay in the appearance of the transformed phenotype indicated that some intracellular event or series of events subsequent to the establishment of transcriptionally active bovine papillomavirus 1 hybrid plasmid is required for the manifestation of the transformed phenotype.
我们描述了一种牛乳头瘤病毒杂交质粒，该质粒含有来自Tn5的新霉素抗性基因，并插入到哺乳动物细胞转录单元中。在选择具有转化表型或对氨基糖苷类G418有抗性的小鼠细胞中，该质粒作为稳定的染色体外元件维持（每个二倍体基因组有20至100个拷贝）。选择对G418有抗性的细胞最初表现出扁平、未转化的表型，然后才展现出转化的总体形态特征。转化表型出现的延迟表明，转录活性牛乳头瘤病毒1杂交质粒建立后，某些细胞内事件或一系列事件是转化表型表现所必需的。
