Sens M A, Garvin A J, Drew S, Smith C D, Othersen H B, Sens D A
Arch Pathol Lab Med. 1984 Jan;108(1):58-62.
We studied a case of Wilms' tumor with focal areas of well-differentiated skeletal muscle by immunoperoxidase staining using anti-skeletal muscle myosin and by tissue culture explant of the tumor. Strong immunostaining occurred in areas of well-differentiated muscle but also occurred in focal areas where the cells were undifferentiated. The primary explant of this tumor was attempted using standard serum-containing culture media and a serum-free hormonally defined culture medium. While serum-containing culture media gave rise to a fibroblastlike cell population, the serum-free hormonally defined medium produced cells of myoid origin. Ultrastructurally, all stages of early myogenesis from myoblasts to myotubules were observed in this primary explant grown in serum-free medium.
我们通过使用抗骨骼肌肌球蛋白的免疫过氧化物酶染色以及肿瘤组织培养外植体研究了一例伴有局灶性高分化骨骼肌区域的肾母细胞瘤。在高分化肌肉区域出现强免疫染色，但在细胞未分化的局灶区域也出现了免疫染色。使用标准含血清培养基和无血清激素限定培养基尝试对该肿瘤进行原代外植体培养。含血清培养基产生了成纤维细胞样细胞群体，而无血清激素限定培养基产生了肌样来源的细胞。在超微结构上，在无血清培养基中生长的该原代外植体中观察到了从成肌细胞到肌管的早期肌生成的各个阶段。
