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用金标记单克隆IgG对轮状病毒组抗原进行电子显微镜鉴定

Electron microscopic identification of rotavirus group antigen with gold-labelled monoclonal IgG.

作者信息

Martin M L, Palmer E L

出版信息

Arch Virol. 1983;78(3-4):279-85. doi: 10.1007/BF01311322.

DOI:10.1007/BF01311322
PMID:6318697
Abstract

Colloidal gold was complexed with monoclonal IgG to the inner capsid of human rotavirus. The complex was then used as an immunoelectron microscopic marker to identify the location of the group-specific antigen of rotavirus.

摘要

将胶体金与针对人轮状病毒内衣壳的单克隆IgG复合。然后将该复合物用作免疫电子显微镜标记物,以鉴定轮状病毒群特异性抗原的位置。

相似文献

1
Electron microscopic identification of rotavirus group antigen with gold-labelled monoclonal IgG.用金标记单克隆IgG对轮状病毒组抗原进行电子显微镜鉴定
Arch Virol. 1983;78(3-4):279-85. doi: 10.1007/BF01311322.
2
Immuno-electron microscopic study on the antigenicity of tubular structures associated with human rotavirus.人轮状病毒相关管状结构抗原性的免疫电子显微镜研究
Infect Immun. 1981 Aug;33(2):611-5. doi: 10.1128/iai.33.2.611-615.1981.
3
Development of a sensitive protein A-gold immunoelectron microscopy method for detecting viral antigens in fluid specimens.
J Virol Methods. 1985 Oct;12(1-2):135-47. doi: 10.1016/0166-0934(85)90014-x.
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Ultrastructural localization of rotavirus antigens using colloidal gold.使用胶体金对轮状病毒抗原进行超微结构定位
Virus Res. 1984;1(2):133-52. doi: 10.1016/0168-1702(84)90069-8.
5
Specific labelling of human rotaviruses and adenoviruses with gold-IgG complexes.
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6
Sensitive solid-phase immune electron microscopy double-antibody technique with gold-immunoglobulin G complexes for detecting rotavirus in cell culture and feces.采用金免疫球蛋白G复合物的敏感固相免疫电子显微镜双抗体技术检测细胞培养物和粪便中的轮状病毒。
J Clin Microbiol. 1990 May;28(5):864-8. doi: 10.1128/jcm.28.5.864-868.1990.
7
Comparison of an enzyme immunoassay with electron microscopic procedures for detecting rotavirus.酶免疫测定法与电子显微镜检测程序在轮状病毒检测中的比较。
J Clin Microbiol. 1982 May;15(5):938-44. doi: 10.1128/jcm.15.5.938-944.1982.
8
The use of markers in immune electron microscopy.
J Virol Methods. 1981 Feb;2(3):169-74. doi: 10.1016/0166-0934(81)90035-5.
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Monoclonal IgG to the inner capsid of human rotavirus.针对人轮状病毒内衣壳的单克隆IgG
Arch Virol. 1982;72(3):223-7. doi: 10.1007/BF01348968.
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[The use of colloidal gold-labelled antibodies for demonstrating viral antigens].[用胶体金标记抗体检测病毒抗原]
Vopr Virusol. 1989 Mar-Apr;34(2):225-30.

本文引用的文献

1
Gold-labelled antibody decoration (GLAD) in the diagnosis of plant viruses by immuno-electron microscopy.免疫电镜中用金标抗体装饰法(GLAD)诊断植物病毒
J Immunol Methods. 1982;51(1):23-8. doi: 10.1016/0022-1759(82)90377-5.
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An electron microscopic demonstration of immune complexes of hepatitis B e-antigen using colloid gold as a marker.
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Sizing of protein A-colloidal gold probes for immunoelectron microscopy.用于免疫电子显微镜的蛋白A-胶体金探针的大小测定
J Cell Biol. 1981 Aug;90(2):533-6. doi: 10.1083/jcb.90.2.533.
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The use of markers in immune electron microscopy.
J Virol Methods. 1981 Feb;2(3):169-74. doi: 10.1016/0166-0934(81)90035-5.
5
Rapid identification of viruses by a simple indirect immune electron microscopy technique using ferritin-labelled antibodies.使用铁蛋白标记抗体的简单间接免疫电子显微镜技术快速鉴定病毒。
J Virol Methods. 1981 Jun;2(6):367-73. doi: 10.1016/0166-0934(81)90060-4.
6
Human rotavirus type 2: cultivation in vitro.人轮状病毒2型:体外培养
Science. 1980 Jan 11;207(4427):189-91. doi: 10.1126/science.6243190.
7
Monoclonal IgG to the inner capsid of human rotavirus.针对人轮状病毒内衣壳的单克隆IgG
Arch Virol. 1982;72(3):223-7. doi: 10.1007/BF01348968.
8
An immunocolloid method for the electron microscope.一种用于电子显微镜的免疫胶体方法。
Immunochemistry. 1971 Nov;8(11):1081-3. doi: 10.1016/0019-2791(71)90496-4.
9
Ultrastructure of infantile gastroenteritis virus.婴儿肠胃炎病毒的超微结构
Virology. 1975 Nov;68(1):146-53. doi: 10.1016/0042-6822(75)90156-7.
10
Characterization of two particle types of calf rotavirus.
J Gen Virol. 1976 May;31(2):245-50. doi: 10.1099/0022-1317-31-2-245.