Akagi J M
Biochem Biophys Res Commun. 1983 Dec 16;117(2):530-5. doi: 10.1016/0006-291x(83)91232-9.
Bisulfite was reduced to sulfide by cell extracts of Desulfotomaculum nigrificans. When trithionate was added to reaction mixtures reducing bisulfite, sulfide formation was inhibited with accumulation of thiosulfate. The thiosulfate reductase activity of cell extracts was found to be inhibited by trithionate. Trithionate alone was reduced to thiosulfate and purified bisulfite reductase (P582) was not affected by trithionate. It is concluded that the pathway for bisulfite reduction in Dt. nigrificans includes trithionate and thiosulfate as intermediate compounds.
黑脱硫肠状菌的细胞提取物可将亚硫酸盐还原为硫化物。当向还原亚硫酸盐的反应混合物中添加连三硫酸盐时，硫化物的形成受到抑制，同时硫代硫酸盐积累。发现细胞提取物的硫代硫酸盐还原酶活性受到连三硫酸盐的抑制。单独的连三硫酸盐可被还原为硫代硫酸盐，而纯化的亚硫酸盐还原酶（P582）不受连三硫酸盐的影响。得出的结论是，黑脱硫肠状菌中还原亚硫酸盐的途径包括连三硫酸盐和硫代硫酸盐作为中间化合物。
