Akagi J M, Chan M, Adams V
J Bacteriol. 1974 Oct;120(1):240-4. doi: 10.1128/jb.120.1.240-244.1974.
The bisulfite reductase (P582) from Desulfotomaculum nigrificans was purified to homogeneity as judged by polyacrylamide gel electrophoresis. By colorimetric methods of analysis, the products of bisulfite reduction by this enzyme were determined to be trithionate, thiosulfate, and sulfide. Of these, trithionate was consistently found to be the major product, whereas the latter two were formed in lesser quantities. When [(35)S]bisulfite was incorporated as substrate, no labeled sulfide was detected. Furthermore, when trithionate and thiosulfate were isolated from reaction mixtures and chemically degraded, (35)S was found in all three sulfur atoms of trithionate; however, only the inner sulfur atom of thiosulfate was radioactive. From these data we conclude that the bisulfite reductase of D. nigrificans reduces bisulfite to trithionate and that thiosulfate and sulfide are endogenous side products of the reaction.
通过聚丙烯酰胺凝胶电泳判断，来自黑化脱硫肠状菌的亚硫酸氢盐还原酶（P582）被纯化至同质。通过比色分析方法，确定该酶催化亚硫酸氢盐还原的产物为连三硫酸盐、硫代硫酸盐和硫化物。其中，连三硫酸盐始终被发现是主要产物，而后两者的生成量较少。当以[（35）S]亚硫酸氢盐作为底物时，未检测到标记的硫化物。此外，当从反应混合物中分离出连三硫酸盐和硫代硫酸盐并进行化学降解时，在连三硫酸盐的所有三个硫原子中都发现了（35）S；然而，只有硫代硫酸盐的内部硫原子具有放射性。根据这些数据，我们得出结论，黑化脱硫肠状菌的亚硫酸氢盐还原酶将亚硫酸氢盐还原为连三硫酸盐，硫代硫酸盐和硫化物是该反应的内源性副产物。
