噬菌体T4基因组早期区域的克隆与物理图谱构建
Cloning and physical mapping of an early region of the bacteriophage T4 genome.
作者信息
Macdonald P M, Mosig G
出版信息
Genetics. 1984 Jan;106(1):1-16. doi: 10.1093/genetics/106.1.1.
Abstract
We have cloned DNA restriction fragments from the largely nonessential region of bacteriophage T4 located between genes 39 and 56. The cloned DNA fragments were used to construct a precise map of the sites in this region recognized by eight restriction endonucleases. This restriction map allowed us to compare the cytosine-containing T4 DNA used for cloning with the hydroxymethylcytosine-containing DNA of wild-type T4; there were no detectable rearrangements in the region tested. We were also able to determine the physical locations of several deletion end points and of several genes.
摘要
我们从噬菌体T4位于基因39和56之间的基本非必需区域克隆了DNA限制片段。克隆的DNA片段用于构建该区域中被8种限制性内切酶识别的位点的精确图谱。该限制图谱使我们能够将用于克隆的含胞嘧啶的T4 DNA与野生型T4的含羟甲基胞嘧啶的DNA进行比较；在测试区域未检测到重排。我们还能够确定几个缺失端点和几个基因的物理位置。
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