Arthur H M, Eastlake P B
Gene. 1983 Nov;25(2-3):309-16. doi: 10.1016/0378-1119(83)90235-4.
Transcription of the uvrD gene of Escherichia coli was studied using the Mud(Aprlac) gene fusion technique of Casadaban and Cohen [Proc. Natl. Acad. Sci. USA 76 (1979) 4530-4533]. Strains were isolated with Mud(Aprlac) inserted in both orientations and chromosome mobilisation experiments showed that transcription of uvrD was from ilvD towards metE. Constitutive expression of uvrD was approximately equivalent to 3000 protein molecules per cell. This level increased 1.5-fold following treatment with DNA damaging agents, an increase which was regulated by the recA and lexA genes. In addition, the constitutive expression of uvrD was reduced in strains containing either the recA56 mutation or a multi-copy plasmid carrying lexA+. These results indicate that uvrD is an SOS-inducible gene.
利用卡萨达班和科恩的Mud（Aprlac）基因融合技术[《美国国家科学院院刊》76（1979）4530 - 4533]研究了大肠杆菌uvrD基因的转录情况。分离出了Mud（Aprlac）以两种方向插入的菌株，染色体迁移实验表明uvrD的转录方向是从ilvD到metE。uvrD的组成型表达大约相当于每个细胞3000个蛋白质分子。在用DNA损伤剂处理后，这个水平增加了1.5倍，这种增加受recA和lexA基因调控。此外，在含有recA56突变或携带lexA + 的多拷贝质粒的菌株中，uvrD的组成型表达降低。这些结果表明uvrD是一个SOS诱导型基因。
