Gündüz U, Katze J R
J Biol Chem. 1984 Jan 25;259(2):1110-3.
Cell-free extracts of the Vero cell line (monkey kidney origin) contain an activity which converts 7-[5-[( (1S,4S,5R)-4, 5-dihydroxy-2-cyclopenten-1-yl)-amino]methyl]-7-deazaguanosine (queuosine) 5'-phosphate into queuine (the base of queuosine). We designate this the queuine salvage activity because it apparently is responsible for the ability of intact Vero cells to salvage queuosine base from tRNA degraded during the normal turnover process. Queuosine-3'-P, mannosylqueuosine-5'-P, or queuosine nucleoside does not support the queuine salvage reaction. Extracts of the L-M cell line (mouse embryo origin) lack the queuine salvage activity, a finding consistent with the inability of intact L-M cells to retrieve queuine subsequent to tRNA turnover (Gündüz, U., and Katze, J. R. (1982) Biochem. Biophys. Res. Commun. 109, 159-167).
非洲绿猴肾细胞系（源自猴肾）的无细胞提取物含有一种活性，可将7-[5-[( (1S,4S,5R)-4,5-二羟基-2-环戊烯-1-基)-氨基]甲基]-7-脱氮鸟苷（queuosine）5'-磷酸转化为queuine（queuosine的碱基）。我们将此称为queuine挽救活性，因为它显然是完整的非洲绿猴肾细胞从正常周转过程中降解的tRNA中挽救queuosine碱基能力的原因。Queuosine-3'-P、甘露糖基queuosine-5'-P或queuosine核苷不支持queuine挽救反应。L-M细胞系（源自小鼠胚胎）的提取物缺乏queuine挽救活性，这一发现与完整的L-M细胞在tRNA周转后无法回收queuine一致（贡杜兹，U.，和卡茨，J.R.（1982年）《生物化学与生物物理学研究通讯》109，159 - 167）。
