Suzuki H, Kurita T, Kakinuma K
Blood. 1982 Aug;60(2):446-53.
Neuraminidase type X (NMD-type-X, Sigma Chemical Co., St. Louis, Mo.), which is obtained from a further purification of neuraminidase type V (NMD-type-V, Sigma), markedly enhanced the release of O2- and H2O2 from phagocytosing human polymorphonuclear leukocytes (PMN). In contrast, O2 consumption by NMD-type-X-treated PMN was identical to that of untreated PMN. Morphological observations suggested that the enhancement of O2- and H2O2 release was caused by excessive release of the oxygen metabolites into the extracellular medium from incompletely formed phagocytic vacuoles as was observed with cytochalasin-B-treated cells. Our observations are in contrast to the previous reports of Tsan et al. that showed complete inhibition of both O2- and H2O2 release from phagocytosing PMN by the treatment with NMD-type-V.
X型神经氨酸酶（NMD-X型，西格玛化学公司，密苏里州圣路易斯），它是从V型神经氨酸酶（NMD-V型，西格玛）的进一步纯化中获得的，可显著增强吞噬作用的人多形核白细胞（PMN）释放O2-和H2O2。相比之下，经NMD-X型处理的PMN的O2消耗量与未处理的PMN相同。形态学观察表明，O2-和H2O2释放的增强是由于氧代谢产物从不完全形成的吞噬泡过度释放到细胞外介质中，这与用细胞松弛素B处理的细胞所观察到的情况相同。我们的观察结果与Tsan等人之前的报道相反，他们的报道显示用NMD-V型处理可完全抑制吞噬PMN释放O2-和H2O2。
