Ozawa Shigeru, Eda Hiroyuki, Ishii Yasuyuki, Ban Fumihiko, Funabashi Toshiyuki, Hata Seiichiro, Hayashi Kozaburo, Iga Hiroki, Ikushima Takao, Ishiko Hiroaki, Itagaki Tomoo, Kawana Rinji, Kobayashi Shunsaku, Ogino Takeo, Sekizawa Tsuyoshi, Shimomura Yoshikazu, Shiota Hiroshi, Mori Ryoichi, Nakakita Takashi, Numazaki Yoshio, Ozaki Yoshikatsu, Yamamoto Shigeru, Yoshino Kamesaburo, Yanagi Kazuo
Herpesvirus Laboratory, Department of Virology I, National Institute of Infectious Diseases, Toyama 1-23-1, Tokyo 162-8640, Japan.
J Clin Microbiol. 2007 Jul;45(7):2183-90. doi: 10.1128/JCM.02472-06. Epub 2007 May 2.
The identification and geographic distribution of the herpes simplex virus type 1 (HSV-1) BglII restriction fragment length polymorphism (RFLP) variants named BgK(L) and BgO(L) in clinical isolates from orolabial and cutaneous sites were described in our previous reports, in which the dispersion and replacement of HSV-1 variants were proposed. The base substitution sites deduced from the BgK(L) multiple RFLP variations were mapped to the U(L)12 (DNase), R(L)2 (alpha0 transactivator), and latency-associated transcript genes in the present study. The results show that the relative frequencies (RFs) of BgK(L) are significantly higher in orolabial and cutaneous HSV-1 infections than in ocular infections. For the BgO(L) variant, the opposite was found; i.e., the RF of BgO(L) was significantly lower in orolabial and cutaneous infections than in ocular infections. No significant differences in the RFs of non-BgK(L):non-BgO(L) isolates were observed. The ratio of the BgK(L) RF to the BgO(L) RF was much higher for the orolabial and cutaneous infection groups than for the ocular infection group, whereas the BgK(L) RF-to-non-BgK(L):non-BgO(L) RF ratios for the former groups were slightly higher than those for the latter group. The higher efficiency of orolabial and cutaneous infections caused by BgK(L) compared to the efficiency of infections caused by BgO(L) allows BgK(L) to spread more efficiently in human populations and to displace BgO(L), because the mouth and lips are the most common HSV-1 infection sites in children. The present study supports our HSV-1 dispersion-and-replacement hypothesis and suggests that HSV-1, the latency-reactivation of which allows variants to accumulate in human populations, has evolved under competitive conditions, providing a new perspective on the polymorphism or variation of HSV-1.
我们之前的报告描述了在口腔唇部和皮肤部位临床分离株中命名为BgK(L)和BgO(L)的单纯疱疹病毒1型（HSV-1）BglII限制性片段长度多态性（RFLP）变体的鉴定及其地理分布，其中提出了HSV-1变体的扩散和替代情况。在本研究中，从BgK(L)多个RFLP变异推导的碱基替换位点被定位到U(L)12（DNase）、R(L)2（α0反式激活因子）和潜伏相关转录基因。结果显示，BgK(L)在口腔唇部和皮肤HSV-1感染中的相对频率（RFs）显著高于眼部感染。对于BgO(L)变体，情况则相反；即BgO(L)在口腔唇部和皮肤感染中的RF显著低于眼部感染。未观察到非BgK(L)：非BgO(L)分离株RFs的显著差异。口腔唇部和皮肤感染组的BgK(L) RF与BgO(L) RF的比值远高于眼部感染组，而前一组的BgK(L) RF与非BgK(L)：非BgO(L) RF的比值略高于后一组。与BgO(L)引起感染的效率相比，BgK(L)引起口腔唇部和皮肤感染的效率更高，这使得BgK(L)能够在人群中更有效地传播并取代BgO(L)，因为口腔和唇部是儿童中最常见的HSV-1感染部位。本研究支持我们的HSV-1扩散和替代假说，并表明HSV-1的潜伏再激活使变体在人群中积累，它是在竞争条件下进化的，为HSV-1的多态性或变异性提供了新视角。
