Gorbacheva I M, Kebekbaeva K M, Bezborodov A M
Mikrobiologiia. 1983 Sep-Oct;52(5):755-60.
The work was aimed at studying the ability of Aspergillus and Penicillium species to isolate phosphomonoesterases (PMEases) in the cultural broth in the course of submerged cultivation in a complex nutrient medium. The cultures produced both acid and alkaline PMEases, but acid PMEase was produced at a much greater rate than alkaline PMEase. The activity of acid and alkaline PMEases decreased in most cases when the cultural broth and cell-free extracts were incubated with urea. The activity of exocellular acid PMEases was inhibited by the cell-free extracts of most cultures. The inhibition of PMEases from different sources is manifested in the studied cultures of Aspergillus and Penicillium in a different manner.
这项工作旨在研究曲霉属和青霉属菌种在复杂营养培养基中进行深层培养过程中，从培养液中分离磷酸单酯酶（PMEases）的能力。这些培养物产生了酸性和碱性PMEases，但酸性PMEase的产生速率比碱性PMEase高得多。在大多数情况下，当培养液和无细胞提取物与尿素一起孵育时，酸性和碱性PMEases的活性会降低。大多数培养物的无细胞提取物会抑制胞外酸性PMEases的活性。在曲霉属和青霉属的研究培养物中，不同来源的PMEases的抑制作用表现方式不同。
