凝胶分析：用Pascal语言实现的一维电泳凝胶分析。

GELYSIS: Pascal-implemented analysis of one-dimensional electrophoresis gels.

作者信息

Russell P J, Crandall R E, Feinbaum R

出版信息

Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):493-8. doi: 10.1093/nar/12.1part2.493.

DOI:10.1093/nar/12.1part2.493

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321065/

Abstract

GELYSIS (GEL analYSIS) is a Pascal implementation of an algorithm that takes data on the distances migrated by DNA fragments in a one-dimension electrophoretic gel and calculates the sizes of the fragments based on the known sizes of marker fragments. A key step in the overall algorithm is a cubic-spline best-fit of marker calibration data. An option of the program is the generation of graphic representations of the gel and of the calibration spline that are convenient for primary data storage or for publication. Provision is made for specifying different band intensities in the graphic output of the gel.

摘要

GELYSIS（凝胶分析）是一种用Pascal语言实现的算法，它获取一维电泳凝胶中DNA片段迁移距离的数据，并根据已知大小的标记片段来计算这些片段的大小。整个算法的关键步骤是对标记校准数据进行三次样条最佳拟合。该程序的一个选项是生成凝胶和校准样条的图形表示，这便于原始数据存储或发表。程序提供了在凝胶图形输出中指定不同条带强度的功能。

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