Kennedy P G, Watkins B A, LaThangue N B, Clements G B, Thomas D G
J Neurooncol. 1987;4(4):389-96. doi: 10.1007/BF00195610.
Cell cultures derived from 60 different human brain tumors were screened for the presence of HSV infected cell antigens by indirect immunofluorescence using a polyclonal rabbit antiserum reacting with herpes simplex virus (HSV), 3 monoclonal antibodies recognising different HSV-specified proteins, and one monoclonal antibody T181 reacting with a DNA binding protein present in HSV-infected cells. Only one tumor (IN/157), derived from an oligodendroglioma, stained with the polyclonal antiserum. T181 but none of the other monoclonal antibodies used also specifically reacted with IN/157 cells. High levels of the T181-defined protein were detected using immunoblotting in HSV-1 infected BHK/21 cells but not in IN/157 cells. T181 may react with either an epitope shared between two different molecules in HSV-1 infected and IN/157 cells or a cell-specified polypeptide that is upregulated after HSV-1 infection.
利用与单纯疱疹病毒（HSV）反应的多克隆兔抗血清、3种识别不同HSV特异性蛋白的单克隆抗体以及一种与HSV感染细胞中存在的DNA结合蛋白反应的单克隆抗体T181，通过间接免疫荧光法对源自60种不同人脑肿瘤的细胞培养物进行筛选，以检测HSV感染细胞抗原的存在。只有一种源自少突胶质细胞瘤的肿瘤（IN/157）被多克隆抗血清染色。T181，但所用的其他单克隆抗体均未与IN/157细胞发生特异性反应。在HSV-1感染的BHK/21细胞中通过免疫印迹检测到高水平的T181定义蛋白，但在IN/157细胞中未检测到。T181可能与HSV-1感染细胞和IN/157细胞中两种不同分子之间共享的表位发生反应，或者与HSV-1感染后上调的细胞特异性多肽发生反应。
