Eigel A, Feldmann H
EMBO J. 1982;1(10):1245-50. doi: 10.1002/j.1460-2075.1982.tb00020.x.
A comparative analysis of a number of yeast DNA-pBR322 recombinant plasmids carrying repetitive sequence elements has revealed that Ty1 or delta elements occur in the vicinity of several tRNA genes. Four examples have been characterized in detail: three glutamate tRNA genes and a serine tRNA gene. The tRNAGlu3 genes occupy different chromosomal locations; two of these genes are found adjacent to Ty1 elements, and the third is found adjacent to an independent delta element. A delta unit is also found adjacent to a tRNASer2 gene. Next to one of the tRNAGlu3 genes, the delta element is joined to a truncated sigma element. Junctions between different delta units were characterized by the sequence analysis of two DNA segments that carry no tRNA genes.
对多个携带重复序列元件的酵母DNA-pBR322重组质粒进行的比较分析表明,Ty1或δ元件出现在多个tRNA基因附近。已详细鉴定了四个例子:三个谷氨酸tRNA基因和一个丝氨酸tRNA基因。tRNAGlu3基因占据不同的染色体位置;其中两个基因位于Ty1元件附近,第三个基因位于一个独立的δ元件附近。在tRNASer2基因附近也发现了一个δ单元。在其中一个tRNAGlu3基因旁边,δ元件与一个截短的σ元件相连。通过对两个不携带tRNA基因的DNA片段进行序列分析,确定了不同δ单元之间的连接。