Dobson M J, Tuite M F, Mellor J, Roberts N A, King R M, Burke D C, Kingsman A J, Kingsman S M
Nucleic Acids Res. 1983 Apr 25;11(8):2287-302. doi: 10.1093/nar/11.8.2287.
The complete 5' flanking region of the yeast TRP1 gene encoding N-(5'-phosphoribosyl)- anthranilate isomerase, a nonabundant protein, has been cloned and the nucleotide sequence data has been extended from -102 to -440. The CT block--CAAG structure common to all efficiently expressed yeast genes is altered in the 5' region of TRP1 and a sequence postulated to be involved in general amino acid regulation is absent. There are two possible TATA boxes at -224 and -262. TRP1, in common with HIS3, HIS4 and TRP5 has a region of dyad symmetry upstream of the coding sequence which may play a role in initiation of transcription. The relative efficiency of gene expression directed by the complete 5' TRP1 region was assessed by comparison with that of PGK by inserting a cDNA for a human interferon-alpha downstream of their respective 5' regions. The respective interferon yields indicate that their in vivo expression capabilities are a function of their 5' regions.
编码N -（5'-磷酸核糖基）-邻氨基苯甲酸异构酶（一种含量不高的蛋白质）的酵母TRP1基因完整的5'侧翼区域已被克隆，核苷酸序列数据已从-102延伸至-440。所有高效表达的酵母基因共有的CT框 - CAAG结构在TRP1的5'区域发生改变，并且推测参与一般氨基酸调节的序列缺失。在-224和-262处有两个可能的TATA框。与HIS3、HIS4和TRP5一样，TRP1在编码序列上游有一个二元对称区域，可能在转录起始中起作用。通过将人α-干扰素的cDNA插入其各自5'区域的下游，与PGK的表达效率进行比较，评估了由完整的5'TRP1区域指导的基因表达的相对效率。各自的干扰素产量表明它们在体内的表达能力是其5'区域的一个函数。
