家蚕核糖体DNA的内含子及其侧翼序列。
Introns and their flanking sequences of Bombyx mori rDNA.
作者信息
Fujiwara H, Ogura T, Takada N, Miyajima N, Ishikawa H, Maekawa H
出版信息
Nucleic Acids Res. 1984 Sep 11;12(17):6861-9. doi: 10.1093/nar/12.17.6861.
Abstract
We obtained two different clones (16 kb and 13 kb) of B. mori rDNA with intron sequence within the 28S-rRNA coding region. The sequence surrounding the intron was found to be highly conserved as indicated in several eukaryotes (Tetrahymena, Drosophila and Xenopus). The 28S rRNA-coding sequence of 16 kb and 13 kb clone was interrupted at precisely the same sites as those where the D. melanogaster rDNA interrupted by the type I and type II intron, respectively. The intron sequences of B. mori were different from those of D. melanogaster. In 16 kb clone, the intron was flanked by 14 bp duplication of the junction sequence, which was also present once within the 28S rRNA-coding region of rDNA without intron. This 14 bp sequence was identical with those surrounding the introns of Dipteran rDNAs.
摘要
我们获得了家蚕核糖体DNA（rDNA）的两个不同克隆（16 kb和13 kb），其28S - rRNA编码区域内含有内含子序列。如在几种真核生物（嗜热四膜虫、果蝇和非洲爪蟾）中所示，内含子周围的序列高度保守。16 kb和13 kb克隆的28S rRNA编码序列分别在与黑腹果蝇rDNA被I型和II型内含子打断的相同位点处中断。家蚕的内含子序列与黑腹果蝇的不同。在16 kb克隆中，内含子两侧是连接序列的14 bp重复，该重复在无内含子的rDNA的28S rRNA编码区域内也出现过一次。这个14 bp序列与双翅目rDNA内含子周围的序列相同。
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