嗜粪Sciara不同核糖体DNA重复单元中的两个不同间隔序列。
Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.
作者信息
Renkawitz-Pohl R, Matsumoto L, Gerbi S A
出版信息
Nucleic Acids Res. 1981 Aug 11;9(15):3747-64. doi: 10.1093/nar/9.15.3747.
We have prepared a partial gene library of sheared DNA from the fungus fly, Sciara coprophila, by dA-T tailing and insertion into pBR322. Two ribosomal DNA clones which differ from the usual ribosomal DNA organization in this organism were studied in detail. Clone pBc 1L-1 has an intervening sequence of 1.4 kb, and clone pBc 6D-6 has an intervening sequence of 0.9 kb. These intervening sequences occur in about the same position in 28S rDNA, but do not appear to share sequence homology with one another. Previously we found that 90% of Sciara ribosomal DNA is homogenous and lacks an intervening sequence, and our present data explains the size heterogeneity found in most of the remaining 10%. We have found no evidence of size heterogeneity in the nontranscribed spacer.
我们通过dA-T加尾并插入pBR322，构建了粪蝇（Sciara coprophila）剪切DNA的部分基因文库。对该生物中两个与通常核糖体DNA组织不同的核糖体DNA克隆进行了详细研究。克隆pBc 1L-1有一个1.4 kb的间隔序列，克隆pBc 6D-6有一个0.9 kb的间隔序列。这些间隔序列出现在28S rDNA的大致相同位置，但彼此之间似乎没有序列同源性。此前我们发现，90%的粪蝇核糖体DNA是同质的，没有间隔序列，我们目前的数据解释了其余10%中大部分所发现的大小异质性。我们在非转录间隔区未发现大小异质性的证据。
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