Subramani S, Southern P J
Anal Biochem. 1983 Nov;135(1):1-15. doi: 10.1016/0003-2697(83)90723-6.
Extensive studies on the DNA tumor virus Simian Virus 40 (SV40) have provided a wealth of information regarding the genome organization, regulation of viral gene expression, and the mechanism of DNA replication. SV40 can grow lytically in permissive monkey cells or the viral DNA can integrate into the host genome of nonpermissive rodent cells causing morphological transformation. The viral DNA exists as a minichromosome within the nuclei of lytically infected cells and, as a consequence of DNA replication, there is a significant amplification of the viral genome during infection. These properties suggested that SV40 could be developed as a transducing vector to introduce exogenous DNA into mammalian cells and to express this foreign DNA during the SV40 infectious cycle. In this article the properties of SV40 virus vectors and SV40 hybrid plasmid vectors are described and contrasted.
对DNA肿瘤病毒猿猴病毒40（SV40）的广泛研究提供了大量有关基因组组织、病毒基因表达调控以及DNA复制机制的信息。SV40可以在允许的猴细胞中进行裂解生长，或者病毒DNA可以整合到非允许的啮齿动物细胞的宿主基因组中，导致形态转化。病毒DNA以微型染色体的形式存在于裂解感染细胞的细胞核中，并且由于DNA复制，在感染期间病毒基因组会有显著扩增。这些特性表明SV40可以被开发为一种转导载体，用于将外源DNA引入哺乳动物细胞，并在SV40感染周期中表达这种外源DNA。在本文中，将描述并对比SV40病毒载体和SV40杂交质粒载体的特性。
